Maria Clara Jessica CalastriSão José do Rio Preto School of Medicine - FAMERP, Brazil
Title: Overexpression VEGF may be regulated by low levels of miR-145-3p and miR-101-3p on cholangiocarcinoma.
Introduction: Cholangiocarcinoma (CCA) is the second most common type of primary liver cancer. Epigenetic changes involving promoter genes, gene expression, and microRNAs (miR) can contribute to genomic instability in cancer. This study aimed to evaluating the expression of VEGF and miRs 145-3p and 101-3p in CCA, its potential as a biomarker for diagnosis and prognosis, as well as its association with comorbidities and lifestyle. Material and Methods: Sixty-two subjects were studied in which 41 cases (study group – CG) had confirmed CCA and 21 individuos with cholelithiasis, as a control group (CG). The RNA was extracted from a paraffined tissue block, and fresh tissue (control group) and the synthesis of cDNA was performed, as well as the analysis of the expression of VEGF, miR-145-3p, and miR-101-3p carried out by polymerase chain reaction in real time. Results: miRs 145-3p and 101-3p were under expressed in SG compared to CG (0.46; 0.17; P=0.0001, respectively). VEGF was overexpressed in the SG compared to CG (11.8; P = 0.0001). An increase in miR-145-3p levels was observed in patients with perihilar (pCCA), versus distal (dCCA) CCA (0.51 ± 0.41; 0.17 ± 0.13, respectively), but not significant (P>0.05). Survival analysis showed 41.9% of patients with intrahepatic CCA free from the event (death) in 11 months, compared to 31.5% for extrahepatic CCA (P>0.05). For life habits, smoking prevailed in SG compared to CG (36.6%, 9.5%; P=0.034). A higher age range was observed in patients with CCA (median=56 years), compared to the CG (median=39 years; P=0.0003). Conclusion: The underexpression of miRNAs, tumor suppressors, and the overexpression of VEGF are associated with CCA, as well as smoking and increasing age. The reduced expression of the referred miRNAs, and increased VEGF can contribute to a decrease in survival, particularly for tumor types in the intrahepatic bile ducts
She is Bachelor in Biology (2012) and has completed her master's degree in Health Sciences from the Faculty of Medicine of São José do Rio Preto - FAMERP (2016). Currently, this researcher is finishing her PhD studies and has worked for 10 years as a collaborating researcher at the Research Center in Biochemistry and Molecular Biology (NPBIM/FAMERP). Has experience in Molecular Biology and Genetics, working mainly on the following subjects: Macular Degeneration, Molecular Characterization of Neurogliomas, Hepatocellular Carcinoma and Cholangiocarcinoma. She has 7 publications, 30 abstracts published in conference proceedings and 45 article presentations. Professor at Uniterp - Colégio Técnico e Faculdade for 6 years and academic coordinator of the Postgraduate Course in Radiology and Radiotherapy (FAMERP).