Judith Marcela Dueñas-jiménezUniversity of Guadalajara, Mexico
Title: GPER expression is regulated by their specific agonist (G1) and antagonist (G15) in C6 Glioblastoma cells in vitro
Glioblastoma is a very aggressive brain tumor, the patients with Glioblastoma have a bad prognosis with only 18 months of survival. Glioblastoma cells express classic estrogen receptors and secrete 17?-estradiol. The membrane-coupled protein G estrogen receptor (GPER) is involved in the growth and apoptosis of several types of cancer. The role of GPER in Glioblastoma cells is partially known. In the present study, we evaluate GPER expression in C6 cells and their participation in cell division and apoptosis. C6 cells were cultured and treated with G1 (10nM), E2(10nM), G1- G15(10nM and 10µM) and G15 (10 µM) alone. The proliferation was evaluated by immunofluorescence using Ki67 antibody and, apoptosis employing Caspase-3 antibody by immunofluorescence method and also by ELISA assay. Furthermore, we quantify the GPER mRNA expression by quantitative transcription polymerase reverse method (PCR). C6 cells immunopositivity to GPER increased by E2, G1, and their combination. The protein expression of GPER rose significantly in the C6 cells treated with E2 +G1. In contrast, GPER mRNA expression decreased in all Glioblastoma treated cells compared to control groups. The percentage of cells in proliferation increased with a significant statistical difference in cells treated with G1 and E2 or their combination. G15 reduced Ki67 immunopositivity cells. We observe a positive Pearson´s correlation between GPER protein expression and KI67 immunopositively cells. Caspase-3 levels increased in C6 cells treated with the antagonist G15. A negative Pearson´s correlation was observed between Caspase-3 and GPER expression. Glioblastoma cell proliferation increases by E2 and G1, while G15 reduced the proliferation and promoting apoptosis. GPER could be used as a biomarker or a target to control the Glioblastoma growth tumor.
Judith M Dueñas Jimenez obtained her PhD degree at the Complutense University of Madrid Spain. Now works as a professor and researcher at the Guadalajara University. She made a sabbatical year (2007) at the Institute Santiago Ramon y Cajal, in Madrid Spain. Her main investigation is about the effects of estrogens in brain cancer (Glioblastoma tumor). She has 40 papers with more of 700 citations with a h-index of 6. Dr. Dueñas is member of the National System of Researchers in Mexico and member of the Society for Neuroscience USA